Transmission of Rat Hepatitis E Virus Infection to Humans in Hong Kong: A Clinical and Epidemiological Analysis

Siddharth Sridhar, Cyril Chik Yan Yip, Shusheng Wu, Nicholas Foo Siong Chew, Kit Hang Leung, Jasper Fuk Woo Chan, Pyrear Suhui Zhao, Wan Mui Chan, Rosana Wing Shan Poon, Hoi Wah Tsoi, Jian Piao Cai, Helen Shuk Ying Chan, Anthony Wai Shing Leung, Cindy Wing Sze Tse, Jonpaul Sze Tsing Zee, Owen Tak Yin Tsang, Vincent Chi Chung Cheng, Susanna Kar Pui Lau, Patrick Chiu Yat Woo, Dominic Ngai Chong TsangKwok Yung Yuen

Research output: Contribution to journalArticlepeer-review

155 Citations (Scopus)

Abstract

Background and Aims: Hepatitis E virus (HEV) variants causing human infection predominantly belong to HEV species A (HEV-A). HEV species C genotype 1 (HEV-C1) circulates in rats and is highly divergent from HEV-A. It was previously considered unable to infect humans, but the first case of human HEV-C1 infection was recently discovered in Hong Kong. The aim of this study is to further describe the features of this zoonosis in Hong Kong. Approach and Results: We conducted a territory-wide prospective screening study for HEV-C1 infection over a 31-month period. Blood samples from 2,860 patients with abnormal liver function (n = 2,201) or immunosuppressive conditions (n = 659) were screened for HEV-C1 RNA. In addition, 186 captured commensal rats were screened for HEV-C1 RNA. Sequences of human-derived and rat-derived HEV-C1 isolates were compared. Epidemiological and clinical features of HEV-C1 infection were analyzed. HEV-C1 RNA was detected in 6/2,201 (0.27%) patients with hepatitis and 1/659 (0.15%) immunocompromised persons. Including the previously reported case, eight HEV-C1 infections were identified, including five in patients who were immunosuppressed. Three patients had acute hepatitis, four had persistent hepatitis, and one had subclinical infection without hepatitis. One patient died of meningoencephalitis, and HEV-C1 was detected in cerebrospinal fluid. HEV-C1 hepatitis was generally milder than HEV-A hepatitis. HEV-C1 RNA was detected in 7/186 (3.76%) rats. One HEV-C1 isolate obtained from a rat captured near the residences of patients was closely related to the major outbreak strain. Conclusions: HEV-C1 is a cause of hepatitis E in humans in Hong Kong. Immunosuppressed individuals are susceptible to persistent HEV-C1 infection and extrahepatic manifestations. Subclinical HEV-C1 infection threatens blood safety. Tests for HEV-C1 are required in clinical laboratories.

Original languageEnglish
Pages (from-to)10-22
Number of pages13
JournalHepatology
Volume73
Issue number1
DOIs
Publication statusPublished - Jan 2021
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2020 by the American Association for the Study of Liver Diseases.

ASJC Scopus Subject Areas

  • Hepatology

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