TY - JOUR
T1 - The use of high-resolution melting analysis for rapid spa typing on methicillin-resistant Staphylococcus aureus clinical isolates
AU - Chen, Jonathan Hon Kwan
AU - Cheng, Vincent Chi Chung
AU - Chan, Jasper Fuk Woo
AU - She, Kevin Kin Kwan
AU - Yan, Mei Kum
AU - Yau, Miranda Chong Yee
AU - Kwan, Grace See Wai
AU - Yam, Wing Cheong
AU - Yuen, Kwok Yung
PY - 2013
Y1 - 2013
N2 - Methicillin-resistant Staphylococcus aureus (MRSA) has been endemic in Hong Kong for three decades. This study evaluated the practical use of high-resolution melting (HRM) real-time PCR analysis on MRSA staphylococcal Protein A (spa) typing on local MRSA isolates. Among 55 clinical MRSA isolates collected in 2011, 12 different spa types were observed by the conventional PCR-sequencing method including the locally predominant spa type t1081 and two locally predominant community acquired MRSA spa types t019 and t437. By using the HRM method, it could differentiate all 12 spa genotypes by distinct melting curves and HRM difference plot analysis. These two methods demonstrated 100% concordance whereas the HRM method required only 3. h of turnaround time and one-fifth of reagent cost compared to the conventional method. Our study confirmed that the cost effective and rapid HRM typing approach is practically useful for MRSA community transmission monitoring and nosocomial outbreak control in Hong Kong.
AB - Methicillin-resistant Staphylococcus aureus (MRSA) has been endemic in Hong Kong for three decades. This study evaluated the practical use of high-resolution melting (HRM) real-time PCR analysis on MRSA staphylococcal Protein A (spa) typing on local MRSA isolates. Among 55 clinical MRSA isolates collected in 2011, 12 different spa types were observed by the conventional PCR-sequencing method including the locally predominant spa type t1081 and two locally predominant community acquired MRSA spa types t019 and t437. By using the HRM method, it could differentiate all 12 spa genotypes by distinct melting curves and HRM difference plot analysis. These two methods demonstrated 100% concordance whereas the HRM method required only 3. h of turnaround time and one-fifth of reagent cost compared to the conventional method. Our study confirmed that the cost effective and rapid HRM typing approach is practically useful for MRSA community transmission monitoring and nosocomial outbreak control in Hong Kong.
KW - Infection control
KW - Methicillin-resistant Staphylococcus aureus
KW - Spa type
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U2 - 10.1016/j.mimet.2012.11.006
DO - 10.1016/j.mimet.2012.11.006
M3 - Article
C2 - 23154043
AN - SCOPUS:84871527526
SN - 0167-7012
VL - 92
SP - 99
EP - 102
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
IS - 2
ER -