TY - JOUR
T1 - Seronegative bacteremic melioidosis caused by Burkholderia pseudomallei with ambiguous biochemical profile
T2 - Clinical importance of accurate identification by 16S rRNA gene and groEL gene sequencing
AU - Woo, Patrick C.Y.
AU - Lau, Susanna K.P.
AU - Woo, Gibson K.S.
AU - Fung, Ami M.Y.
AU - Ngan, Antonio H.Y.
AU - Hui, Wai Ting
AU - Yuen, Kwok Yung
PY - 2003/8/1
Y1 - 2003/8/1
N2 - An aerobic gram-negative bacterium was isolated from the blood and sputum of an 84-year-old, chair-bound nursing home resident with acute bacteremic pneumonia. Although the phenotypic characteristics suggested that the bacterium could be Burkholderia pseudomallei, the Vitek 1 system (GNI+), which can successfully identify 99% of B. pseudomallei strains, showed that the bacterium was "unidentified." Immunoglobulin G against the lipopolysaccharide (LPS) of B. pseudomallei, as detected by an LPS-based enzyme-linked immunosorbent assay with 95% sensitivity, was negative in both the acute-phase and convalescent-phase sera. Sequencing of the groEL gene showed that the isolate was B. pseudomallei. Proper identification of the bacterium in this study is crucial, since there would be a radical difference in the duration of antimicrobial therapy.
AB - An aerobic gram-negative bacterium was isolated from the blood and sputum of an 84-year-old, chair-bound nursing home resident with acute bacteremic pneumonia. Although the phenotypic characteristics suggested that the bacterium could be Burkholderia pseudomallei, the Vitek 1 system (GNI+), which can successfully identify 99% of B. pseudomallei strains, showed that the bacterium was "unidentified." Immunoglobulin G against the lipopolysaccharide (LPS) of B. pseudomallei, as detected by an LPS-based enzyme-linked immunosorbent assay with 95% sensitivity, was negative in both the acute-phase and convalescent-phase sera. Sequencing of the groEL gene showed that the isolate was B. pseudomallei. Proper identification of the bacterium in this study is crucial, since there would be a radical difference in the duration of antimicrobial therapy.
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U2 - 10.1128/JCM.41.8.3973-3977.2003
DO - 10.1128/JCM.41.8.3973-3977.2003
M3 - Article
C2 - 12904433
AN - SCOPUS:0042022029
SN - 0095-1137
VL - 41
SP - 3973
EP - 3977
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 8
ER -