Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis

Patrick C.Y. Woo; Shirley S.L. Ma; Jade L.L. Teng; Maria W.S. Li; Susanna K.P. Lau; Kwok Yung Yuen

doi:10.1007/s10529-007-9447-x

Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis

Patrick C.Y. Woo, Shirley S.L. Ma, Jade L.L. Teng, Maria W.S. Li, Susanna K.P. Lau, Kwok Yung Yuen

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Among 21 human strains of Laribacter hongkongensis, small plasmids were observed in four strains, and large ones in six strains. The smallest, 3264-bp plasmid, pHLHK19, has only one ORF that encodes a putative replication initiator protein and a predicted origin of replication (ori) with a DnaA box, three 18-bp direct repeats and five pairs of inverted repeats. An Escherichia coli-L. hongkongensis shuttle vector was constructed by ligating the HindIII-digested pHLHK19, containing the replication initiator protein and ori of pHLHK19, to HindIII-digested pBK-CMV. This shuttle vector can propagate in E. coli and L. hongkongensis with good transformation efficiencies.

Original language	English
Pages (from-to)	1575-1582
Number of pages	8
Journal	Biotechnology Letters
Volume	29
Issue number	10
DOIs	https://doi.org/10.1007/s10529-007-9447-x
Publication status	Published - Oct 2007
Externally published	Yes

ASJC Scopus Subject Areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Keywords

Laribacter hongkongensis
Plasmid profile
Sequence
Shuttle vector
Transformation

Access to Document

10.1007/s10529-007-9447-x

Cite this

@article{bd42b5fdc4ca451b97c9f88d2568e673,

title = "Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis",

abstract = "Among 21 human strains of Laribacter hongkongensis, small plasmids were observed in four strains, and large ones in six strains. The smallest, 3264-bp plasmid, pHLHK19, has only one ORF that encodes a putative replication initiator protein and a predicted origin of replication (ori) with a DnaA box, three 18-bp direct repeats and five pairs of inverted repeats. An Escherichia coli-L. hongkongensis shuttle vector was constructed by ligating the HindIII-digested pHLHK19, containing the replication initiator protein and ori of pHLHK19, to HindIII-digested pBK-CMV. This shuttle vector can propagate in E. coli and L. hongkongensis with good transformation efficiencies.",

keywords = "Laribacter hongkongensis, Plasmid profile, Sequence, Shuttle vector, Transformation",

author = "Woo, {Patrick C.Y.} and Ma, {Shirley S.L.} and Teng, {Jade L.L.} and Li, {Maria W.S.} and Lau, {Susanna K.P.} and Yuen, {Kwok Yung}",

year = "2007",

month = oct,

doi = "10.1007/s10529-007-9447-x",

language = "English",

volume = "29",

pages = "1575--1582",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Netherlands",

number = "10",

}

TY - JOUR

T1 - Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis

AU - Woo, Patrick C.Y.

AU - Ma, Shirley S.L.

AU - Teng, Jade L.L.

AU - Li, Maria W.S.

AU - Lau, Susanna K.P.

AU - Yuen, Kwok Yung

PY - 2007/10

Y1 - 2007/10

N2 - Among 21 human strains of Laribacter hongkongensis, small plasmids were observed in four strains, and large ones in six strains. The smallest, 3264-bp plasmid, pHLHK19, has only one ORF that encodes a putative replication initiator protein and a predicted origin of replication (ori) with a DnaA box, three 18-bp direct repeats and five pairs of inverted repeats. An Escherichia coli-L. hongkongensis shuttle vector was constructed by ligating the HindIII-digested pHLHK19, containing the replication initiator protein and ori of pHLHK19, to HindIII-digested pBK-CMV. This shuttle vector can propagate in E. coli and L. hongkongensis with good transformation efficiencies.

AB - Among 21 human strains of Laribacter hongkongensis, small plasmids were observed in four strains, and large ones in six strains. The smallest, 3264-bp plasmid, pHLHK19, has only one ORF that encodes a putative replication initiator protein and a predicted origin of replication (ori) with a DnaA box, three 18-bp direct repeats and five pairs of inverted repeats. An Escherichia coli-L. hongkongensis shuttle vector was constructed by ligating the HindIII-digested pHLHK19, containing the replication initiator protein and ori of pHLHK19, to HindIII-digested pBK-CMV. This shuttle vector can propagate in E. coli and L. hongkongensis with good transformation efficiencies.

KW - Laribacter hongkongensis

KW - Plasmid profile

KW - Sequence

KW - Shuttle vector

KW - Transformation

UR - http://www.scopus.com/inward/record.url?scp=34548119182&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548119182&partnerID=8YFLogxK

U2 - 10.1007/s10529-007-9447-x

DO - 10.1007/s10529-007-9447-x

M3 - Article

C2 - 17609856

AN - SCOPUS:34548119182

SN - 0141-5492

VL - 29

SP - 1575

EP - 1582

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 10

ER -

Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis

Abstract

ASJC Scopus Subject Areas

Keywords

Access to Document

Other files and links

Cite this