Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus

Ming Liang He; Bo jian Zheng; Ying Chen; Kin Ling Wong; Jian Dong Huang; Marie C. Lin; Ying Peng; Kwok Y. Yuen; Joseph J.Y. Sung; Hsiang fu Kung

doi:10.1016/j.febslet.2006.03.066

Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus

Ming Liang He, Bo jian Zheng, Ying Chen, Kin Ling Wong, Jian Dong Huang, Marie C. Lin, Ying Peng, Kwok Y. Yuen, Joseph J.Y. Sung, Hsiang fu Kung

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

SARS-associated coronavirus was identified as the etiological agent of severe acute respiratory syndrome and a large virus pool was identified in wild animals. Virus generates drug resistance through fast mutagenesis and escapes antiviral treatment. siRNAs targeting different genes would be an alternative for overcoming drug resistance. Here, we report effective siRNAs targeting structural genes (i.e., spike, envelope, membrane, and nucleocapsid) and their antiviral kinetics. We also showed the synergistic effects of two siRNAs targeting different functional genes at a very low dose. Our findings may pave a way to develop cost effective siRNA agents for antiviral therapy in the future.

Original language	English
Pages (from-to)	2414-2420
Number of pages	7
Journal	FEBS Letters
Volume	580
Issue number	10
DOIs	https://doi.org/10.1016/j.febslet.2006.03.066
Publication status	Published - May 1 2006
Externally published	Yes

ASJC Scopus Subject Areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Keywords

Inhibition of SCoV reproduction
RNA interference
SARS-associated coronavirus
Viral kinetics
siRNA

Access to Document

10.1016/j.febslet.2006.03.066

Cite this

@article{2d74a0e330944cc0b65618cb2b6ce13d,

title = "Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus",

abstract = "SARS-associated coronavirus was identified as the etiological agent of severe acute respiratory syndrome and a large virus pool was identified in wild animals. Virus generates drug resistance through fast mutagenesis and escapes antiviral treatment. siRNAs targeting different genes would be an alternative for overcoming drug resistance. Here, we report effective siRNAs targeting structural genes (i.e., spike, envelope, membrane, and nucleocapsid) and their antiviral kinetics. We also showed the synergistic effects of two siRNAs targeting different functional genes at a very low dose. Our findings may pave a way to develop cost effective siRNA agents for antiviral therapy in the future.",

keywords = "Inhibition of SCoV reproduction, RNA interference, SARS-associated coronavirus, Viral kinetics, siRNA",

author = "He, {Ming Liang} and Zheng, {Bo jian} and Ying Chen and Wong, {Kin Ling} and Huang, {Jian Dong} and Lin, {Marie C.} and Ying Peng and Yuen, {Kwok Y.} and Sung, {Joseph J.Y.} and Kung, {Hsiang fu}",

year = "2006",

month = may,

day = "1",

doi = "10.1016/j.febslet.2006.03.066",

language = "English",

volume = "580",

pages = "2414--2420",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "10",

}

TY - JOUR

T1 - Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus

AU - He, Ming Liang

AU - Zheng, Bo jian

AU - Chen, Ying

AU - Wong, Kin Ling

AU - Huang, Jian Dong

AU - Lin, Marie C.

AU - Peng, Ying

AU - Yuen, Kwok Y.

AU - Sung, Joseph J.Y.

AU - Kung, Hsiang fu

PY - 2006/5/1

Y1 - 2006/5/1

N2 - SARS-associated coronavirus was identified as the etiological agent of severe acute respiratory syndrome and a large virus pool was identified in wild animals. Virus generates drug resistance through fast mutagenesis and escapes antiviral treatment. siRNAs targeting different genes would be an alternative for overcoming drug resistance. Here, we report effective siRNAs targeting structural genes (i.e., spike, envelope, membrane, and nucleocapsid) and their antiviral kinetics. We also showed the synergistic effects of two siRNAs targeting different functional genes at a very low dose. Our findings may pave a way to develop cost effective siRNA agents for antiviral therapy in the future.

AB - SARS-associated coronavirus was identified as the etiological agent of severe acute respiratory syndrome and a large virus pool was identified in wild animals. Virus generates drug resistance through fast mutagenesis and escapes antiviral treatment. siRNAs targeting different genes would be an alternative for overcoming drug resistance. Here, we report effective siRNAs targeting structural genes (i.e., spike, envelope, membrane, and nucleocapsid) and their antiviral kinetics. We also showed the synergistic effects of two siRNAs targeting different functional genes at a very low dose. Our findings may pave a way to develop cost effective siRNA agents for antiviral therapy in the future.

KW - Inhibition of SCoV reproduction

KW - RNA interference

KW - SARS-associated coronavirus

KW - Viral kinetics

KW - siRNA

UR - http://www.scopus.com/inward/record.url?scp=33646092274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646092274&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2006.03.066

DO - 10.1016/j.febslet.2006.03.066

M3 - Article

C2 - 16638566

AN - SCOPUS:33646092274

SN - 0014-5793

VL - 580

SP - 2414

EP - 2420

JO - FEBS Letters

JF - FEBS Letters

IS - 10

ER -

Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus

Abstract

ASJC Scopus Subject Areas

Keywords

Access to Document

Other files and links

Cite this