TY - JOUR
T1 - Identification of acinetobacters on blood agar in presence of D- glucose by unique browning effect
AU - Siau, H.
AU - Yuen, K. Y.
AU - Ho, P. L.
AU - Luk, W. K.
AU - Wong, S. S.Y.
AU - Woo, P. C.Y.
AU - Lee, R. A.
AU - Hui, W. T.
PY - 1998
Y1 - 1998
N2 - A positive phenotypic characteristic of glucose-oxidizing acinetobacters was demonstrated with blood agar containing D-glucose. Glucose-oxidizing Acinetobacter baumannii, Acinetobacter genospecies 3, Acinetobacter lwoffii, and Acinetobacter genospecies 13 sensu Tjernberg and Ursing caused a unique brown discoloration of media supplemented with 5% blood (of horse, sheep, or human origin) and an aldose sugar (0.22 M D- glucose, D-galactose, D-mannose, D-xylose, or lactose). The browning effect was not observed when a ketose sugar (D-fructose or sucrose) was substituted for the aldose sugar or under high osmolarity in the presence of mannitol, glycerol, or sodium chloride. Other gram-negative nonfermenters (non-gluco- oxidizing acinetobacters, Pseudomonas aeruginosa, other Pseudomonas spp., Stenotrophomonas maltophilia, Flavobacterium spp, and Moraxella spp.) did not cause similar discoloration. This novel browning elect may serve as an alternative trait for identifying glucose-oxidizing acinetobacters.
AB - A positive phenotypic characteristic of glucose-oxidizing acinetobacters was demonstrated with blood agar containing D-glucose. Glucose-oxidizing Acinetobacter baumannii, Acinetobacter genospecies 3, Acinetobacter lwoffii, and Acinetobacter genospecies 13 sensu Tjernberg and Ursing caused a unique brown discoloration of media supplemented with 5% blood (of horse, sheep, or human origin) and an aldose sugar (0.22 M D- glucose, D-galactose, D-mannose, D-xylose, or lactose). The browning effect was not observed when a ketose sugar (D-fructose or sucrose) was substituted for the aldose sugar or under high osmolarity in the presence of mannitol, glycerol, or sodium chloride. Other gram-negative nonfermenters (non-gluco- oxidizing acinetobacters, Pseudomonas aeruginosa, other Pseudomonas spp., Stenotrophomonas maltophilia, Flavobacterium spp, and Moraxella spp.) did not cause similar discoloration. This novel browning elect may serve as an alternative trait for identifying glucose-oxidizing acinetobacters.
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U2 - 10.1128/jcm.36.5.1404-1407.1998
DO - 10.1128/jcm.36.5.1404-1407.1998
M3 - Article
C2 - 9574714
AN - SCOPUS:0031969810
SN - 0095-1137
VL - 36
SP - 1404
EP - 1407
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 5
ER -