Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9

Wei Wang; Lei Wei; Anqi Yang; Teng He; K. Y. Yuen; Cheng Chen; Zihe Rao

doi:10.1107/S1744309109014055

Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9

Wei Wang, Lei Wei, Anqi Yang, Teng He, K. Y. Yuen, Cheng Chen, Zihe Rao

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 Å resolution and belonged to space group P21212, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 Å, = Β = = 90° and two molecules per asymmetric unit.

Original language	English
Pages (from-to)	526-528
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	65
Issue number	5
DOIs	https://doi.org/10.1107/S1744309109014055
Publication status	Published - 2009
Externally published	Yes

ASJC Scopus Subject Areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

Keywords

Coronaviruses
Human coronavirus HKU1
Nonstructural proteins

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10.1107/S1744309109014055

Cite this

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abstract = "Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 {\AA} resolution and belonged to space group P21212, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 {\AA}, = Β = = 90° and two molecules per asymmetric unit.",

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AU - Wang, Wei

AU - Wei, Lei

AU - Yang, Anqi

AU - He, Teng

AU - Yuen, K. Y.

AU - Chen, Cheng

AU - Rao, Zihe

PY - 2009

Y1 - 2009

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AB - Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 Å resolution and belonged to space group P21212, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 Å, = Β = = 90° and two molecules per asymmetric unit.

KW - Coronaviruses

KW - Human coronavirus HKU1

KW - Nonstructural proteins

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Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9

Abstract

ASJC Scopus Subject Areas

Keywords

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