Evaluation of NucliSens EasyQ™ HIV-1 assay for quantification of HIV-1 subtypes prevalent in South-east Asia

H. Y. Lam; J. H.K. Chen; K. H. Wong; K. Chan; P. Li; M. P. Lee; D. N. Tsang; K. Y. Yuen; W. C. Yam

doi:10.1016/j.jcv.2006.10.002

Evaluation of NucliSens EasyQ™ HIV-1 assay for quantification of HIV-1 subtypes prevalent in South-east Asia

H. Y. Lam, J. H.K. Chen, K. H. Wong, K. Chan, P. Li, M. P. Lee, D. N. Tsang, K. Y. Yuen, W. C. Yam

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Background: Monitoring anti-retroviral therapy requires that viral load assays for human immunodeficiency virus type 1 (HIV-1) be applicable to diverse HIV-1 subtypes. Objectives: To evaluate NucliSens EasyQ™ HIV-1 assay for quantitation of common HIV-1 subtypes prevalent in South-east Asia. Study design: One hundred and nineteen plasma samples collected in Hong Kong and Cambodia were used to compare the performance of NucliSens EasyQ™ HIV-1 and COBAS Amplicor™ HIV-1 Monitor version 1.5 assays. Viral RNA extracted from the NucliSens MiniMAG™ was also used for HIV-1 subtyping. Results: Performance of NucliSens EasyQ™ correlated well with COBAS Amplicor™ (r = 0.777, p < 0.001) and the small mean difference (0.0462 log₁₀ IU/mL) obtained in the Bland and Altman model indicated good agreement between two assays. The NucliSens EasyQ™ assay demonstrated a 95% sensitivity at 500 IU/mL and 100% specificity. Reproducibility of this assay was within log₁₀ 2-4 IU/mL and had a coefficient of variation between 2.3% and 10.4%. Among the 109 specimens included in the analysis, HIV-1 subtyping identified 64 CRF01_AE, 38 subtype B, 3 subtype C, 3 CRF07_BC and 1 subtype G viruses. Conclusions: Performance of NucliSens EasyQ™ was comparable to COBAS Amplicor™ for HIV-1 viral load monitoring. RNA extracts from NucliSens MiniMAG™ could be used for HIV-1 viral load monitoring, subtyping and drug resistance mutations detection. Our findings highlight the versatility of both NucliSens EasyQ™ and COBAS Amplicor™ in monitoring prevalent subtypes and rare circulating recombinant forms (CRFs) in the South-east Asia region.

Original language	English
Pages (from-to)	39-43
Number of pages	5
Journal	Journal of Clinical Virology
Volume	38
Issue number	1
DOIs	https://doi.org/10.1016/j.jcv.2006.10.002
Publication status	Published - Jan 2007
Externally published	Yes

ASJC Scopus Subject Areas

Virology
Infectious Diseases

Keywords

COBAS Amplicor™
HIV-1
NucliSens EasyQ™
Subtype diversity
Viral load monitoring

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10.1016/j.jcv.2006.10.002

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@article{cf169ee2902241bc806ef72de51a692f,

title = "Evaluation of NucliSens EasyQ{\texttrademark} HIV-1 assay for quantification of HIV-1 subtypes prevalent in South-east Asia",

abstract = "Background: Monitoring anti-retroviral therapy requires that viral load assays for human immunodeficiency virus type 1 (HIV-1) be applicable to diverse HIV-1 subtypes. Objectives: To evaluate NucliSens EasyQ{\texttrademark} HIV-1 assay for quantitation of common HIV-1 subtypes prevalent in South-east Asia. Study design: One hundred and nineteen plasma samples collected in Hong Kong and Cambodia were used to compare the performance of NucliSens EasyQ{\texttrademark} HIV-1 and COBAS Amplicor{\texttrademark} HIV-1 Monitor version 1.5 assays. Viral RNA extracted from the NucliSens MiniMAG{\texttrademark} was also used for HIV-1 subtyping. Results: Performance of NucliSens EasyQ{\texttrademark} correlated well with COBAS Amplicor{\texttrademark} (r = 0.777, p < 0.001) and the small mean difference (0.0462 log10 IU/mL) obtained in the Bland and Altman model indicated good agreement between two assays. The NucliSens EasyQ{\texttrademark} assay demonstrated a 95% sensitivity at 500 IU/mL and 100% specificity. Reproducibility of this assay was within log10 2-4 IU/mL and had a coefficient of variation between 2.3% and 10.4%. Among the 109 specimens included in the analysis, HIV-1 subtyping identified 64 CRF01_AE, 38 subtype B, 3 subtype C, 3 CRF07_BC and 1 subtype G viruses. Conclusions: Performance of NucliSens EasyQ{\texttrademark} was comparable to COBAS Amplicor{\texttrademark} for HIV-1 viral load monitoring. RNA extracts from NucliSens MiniMAG{\texttrademark} could be used for HIV-1 viral load monitoring, subtyping and drug resistance mutations detection. Our findings highlight the versatility of both NucliSens EasyQ{\texttrademark} and COBAS Amplicor{\texttrademark} in monitoring prevalent subtypes and rare circulating recombinant forms (CRFs) in the South-east Asia region.",

keywords = "COBAS Amplicor{\texttrademark}, HIV-1, NucliSens EasyQ{\texttrademark}, Subtype diversity, Viral load monitoring",

author = "Lam, {H. Y.} and Chen, {J. H.K.} and Wong, {K. H.} and K. Chan and P. Li and Lee, {M. P.} and Tsang, {D. N.} and Yuen, {K. Y.} and Yam, {W. C.}",

year = "2007",

month = jan,

doi = "10.1016/j.jcv.2006.10.002",

language = "English",

volume = "38",

pages = "39--43",

journal = "Journal of Clinical Virology",

issn = "1386-6532",

publisher = "Elsevier B.V.",

number = "1",

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TY - JOUR

T1 - Evaluation of NucliSens EasyQ™ HIV-1 assay for quantification of HIV-1 subtypes prevalent in South-east Asia

AU - Lam, H. Y.

AU - Chen, J. H.K.

AU - Wong, K. H.

AU - Chan, K.

AU - Li, P.

AU - Lee, M. P.

AU - Tsang, D. N.

AU - Yuen, K. Y.

AU - Yam, W. C.

PY - 2007/1

Y1 - 2007/1

N2 - Background: Monitoring anti-retroviral therapy requires that viral load assays for human immunodeficiency virus type 1 (HIV-1) be applicable to diverse HIV-1 subtypes. Objectives: To evaluate NucliSens EasyQ™ HIV-1 assay for quantitation of common HIV-1 subtypes prevalent in South-east Asia. Study design: One hundred and nineteen plasma samples collected in Hong Kong and Cambodia were used to compare the performance of NucliSens EasyQ™ HIV-1 and COBAS Amplicor™ HIV-1 Monitor version 1.5 assays. Viral RNA extracted from the NucliSens MiniMAG™ was also used for HIV-1 subtyping. Results: Performance of NucliSens EasyQ™ correlated well with COBAS Amplicor™ (r = 0.777, p < 0.001) and the small mean difference (0.0462 log10 IU/mL) obtained in the Bland and Altman model indicated good agreement between two assays. The NucliSens EasyQ™ assay demonstrated a 95% sensitivity at 500 IU/mL and 100% specificity. Reproducibility of this assay was within log10 2-4 IU/mL and had a coefficient of variation between 2.3% and 10.4%. Among the 109 specimens included in the analysis, HIV-1 subtyping identified 64 CRF01_AE, 38 subtype B, 3 subtype C, 3 CRF07_BC and 1 subtype G viruses. Conclusions: Performance of NucliSens EasyQ™ was comparable to COBAS Amplicor™ for HIV-1 viral load monitoring. RNA extracts from NucliSens MiniMAG™ could be used for HIV-1 viral load monitoring, subtyping and drug resistance mutations detection. Our findings highlight the versatility of both NucliSens EasyQ™ and COBAS Amplicor™ in monitoring prevalent subtypes and rare circulating recombinant forms (CRFs) in the South-east Asia region.

AB - Background: Monitoring anti-retroviral therapy requires that viral load assays for human immunodeficiency virus type 1 (HIV-1) be applicable to diverse HIV-1 subtypes. Objectives: To evaluate NucliSens EasyQ™ HIV-1 assay for quantitation of common HIV-1 subtypes prevalent in South-east Asia. Study design: One hundred and nineteen plasma samples collected in Hong Kong and Cambodia were used to compare the performance of NucliSens EasyQ™ HIV-1 and COBAS Amplicor™ HIV-1 Monitor version 1.5 assays. Viral RNA extracted from the NucliSens MiniMAG™ was also used for HIV-1 subtyping. Results: Performance of NucliSens EasyQ™ correlated well with COBAS Amplicor™ (r = 0.777, p < 0.001) and the small mean difference (0.0462 log10 IU/mL) obtained in the Bland and Altman model indicated good agreement between two assays. The NucliSens EasyQ™ assay demonstrated a 95% sensitivity at 500 IU/mL and 100% specificity. Reproducibility of this assay was within log10 2-4 IU/mL and had a coefficient of variation between 2.3% and 10.4%. Among the 109 specimens included in the analysis, HIV-1 subtyping identified 64 CRF01_AE, 38 subtype B, 3 subtype C, 3 CRF07_BC and 1 subtype G viruses. Conclusions: Performance of NucliSens EasyQ™ was comparable to COBAS Amplicor™ for HIV-1 viral load monitoring. RNA extracts from NucliSens MiniMAG™ could be used for HIV-1 viral load monitoring, subtyping and drug resistance mutations detection. Our findings highlight the versatility of both NucliSens EasyQ™ and COBAS Amplicor™ in monitoring prevalent subtypes and rare circulating recombinant forms (CRFs) in the South-east Asia region.

KW - COBAS Amplicor™

KW - HIV-1

KW - NucliSens EasyQ™

KW - Subtype diversity

KW - Viral load monitoring

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JF - Journal of Clinical Virology

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Evaluation of NucliSens EasyQ™ HIV-1 assay for quantification of HIV-1 subtypes prevalent in South-east Asia

Abstract

ASJC Scopus Subject Areas

Keywords

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