TY - JOUR
T1 - Escherichia coli producing CTX-M β-lactamases in food animals in Hong Kong
AU - Duan, R. S.
AU - Sit, Thomas H.C.
AU - Wong, Samson S.Y.
AU - Wong, River C.W.
AU - Chow, K. H.
AU - Mak, Gannon C.
AU - Ng, L. T.
AU - Yam, W. C.
AU - Yuen, K. Y.
AU - Ho, P. L.
PY - 2006/6
Y1 - 2006/6
N2 - A study was conducted to evaluate the occurrence of extended-spectrum β-lactamases (ESBLs)-producing Escherichia coli from fecal samples of healthy food animals in Hong Kong. Rectal or cloacal swabs were obtained from cattle, pigs, chicken, ducks, geese, and pigeons in slaughterhouses or wholesale markets over a 5-month period in 2002. Antibiotic-containing medium was used for selective isolation of potentially ESBL-producing E. coli. Of 734 samples analyzed, six (2%) from pigs, three (3.1%) from cattle, and one (3%) from pigeons had E. coli strains with the ESBL phenotype. The ESBL content for the 10 isolates include CTX-M-3 (n = 4), CTX-M-13 (n = 3), CTX-M-14 (n = 2), and CTX-M-24 (n = 1). In five isolates, the blaCTX-M gene was encoded on transferable plasmids (60 or 90 kb), and the gene was found to transfer to E. coli (J53 or JP995) with frequencies of 10-7 to 10-3 per donor cells. The ten isolates had five distinct pulsotypes with some clonal spread. However, the isolates from the different kinds of animals were not clonaly related. These findings imply that bacteria of animal origins may serve as reservoirs of some ESBL genes.
AB - A study was conducted to evaluate the occurrence of extended-spectrum β-lactamases (ESBLs)-producing Escherichia coli from fecal samples of healthy food animals in Hong Kong. Rectal or cloacal swabs were obtained from cattle, pigs, chicken, ducks, geese, and pigeons in slaughterhouses or wholesale markets over a 5-month period in 2002. Antibiotic-containing medium was used for selective isolation of potentially ESBL-producing E. coli. Of 734 samples analyzed, six (2%) from pigs, three (3.1%) from cattle, and one (3%) from pigeons had E. coli strains with the ESBL phenotype. The ESBL content for the 10 isolates include CTX-M-3 (n = 4), CTX-M-13 (n = 3), CTX-M-14 (n = 2), and CTX-M-24 (n = 1). In five isolates, the blaCTX-M gene was encoded on transferable plasmids (60 or 90 kb), and the gene was found to transfer to E. coli (J53 or JP995) with frequencies of 10-7 to 10-3 per donor cells. The ten isolates had five distinct pulsotypes with some clonal spread. However, the isolates from the different kinds of animals were not clonaly related. These findings imply that bacteria of animal origins may serve as reservoirs of some ESBL genes.
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U2 - 10.1089/mdr.2006.12.145
DO - 10.1089/mdr.2006.12.145
M3 - Article
C2 - 16922633
AN - SCOPUS:33746712211
SN - 1076-6294
VL - 12
SP - 145
EP - 148
JO - Microbial Drug Resistance
JF - Microbial Drug Resistance
IS - 2
ER -