TY - JOUR
T1 - Development and evaluation of novel real-time reverse transcription-PCR assays with locked nucleic acid probes targeting leader sequences of human-pathogenic coronaviruses
AU - Chan, Jasper Fuk Woo
AU - Choi, Garnet Kwan Yue
AU - Tsang, Alan Ka Lun
AU - Tee, Kah Meng
AU - Lam, Ho Yin
AU - Yip, Cyril Chik Yan
AU - To, Kelvin Kai Wang
AU - Cheng, Vincent Chi Chung
AU - Yeung, Man Lung
AU - Lau, Susanna Kar Pui
AU - Woo, Patrick Chiu Yat
AU - Chan, Kwok Hung
AU - Tang, Bone Siu Fai
AU - Yuen, Kwok Yung
N1 - Publisher Copyright:
Copyright © 2015, American Society for Microbiology. All Rights Reserved.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.
AB - Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.
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UR - http://www.scopus.com/inward/citedby.url?scp=84938100010&partnerID=8YFLogxK
U2 - 10.1128/JCM.01224-15
DO - 10.1128/JCM.01224-15
M3 - Article
C2 - 26019210
AN - SCOPUS:84938100010
SN - 0095-1137
VL - 53
SP - 2722
EP - 2726
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 8
ER -