Cloning and expression of class A β-lactamase gene blaABPS in Burkholderia pseudomallei

Terence K.M. Cheung; P. L. Ho; Patrick C.Y. Woo; K. Y. Yuen; P. Y. Chau

doi:10.1128/AAC.46.4.1132-1135.2002

Cloning and expression of class A β-lactamase gene blaA_BPS in Burkholderia pseudomallei

Terence K.M. Cheung, P. L. Ho, Patrick C.Y. Woo, K. Y. Yuen, P. Y. Chau

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

The β-lactamase gene blaA_BPS in Burkholderia pseudomallei was cloned and expressed in Escherichia coli. BPS-1 is a cephalosporinase with an isoelectric point of 7.7. Sequence analysis of BPS-1 revealed conserved motifs typical of class A β-lactamases and a relationship to the PenA (in B. cepacia) and BlaI (in Yersinia enterocolitica) lineages.

Original language	English
Pages (from-to)	1132-1135
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	46
Issue number	4
DOIs	https://doi.org/10.1128/AAC.46.4.1132-1135.2002
Publication status	Published - 2002
Externally published	Yes

ASJC Scopus Subject Areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

Access to Document

10.1128/AAC.46.4.1132-1135.2002

Cite this

@article{59a345052cdd4ef59aaa7e5de7c6d17f,

title = "Cloning and expression of class A β-lactamase gene blaABPS in Burkholderia pseudomallei",

abstract = "The β-lactamase gene blaABPS in Burkholderia pseudomallei was cloned and expressed in Escherichia coli. BPS-1 is a cephalosporinase with an isoelectric point of 7.7. Sequence analysis of BPS-1 revealed conserved motifs typical of class A β-lactamases and a relationship to the PenA (in B. cepacia) and BlaI (in Yersinia enterocolitica) lineages.",

author = "Cheung, {Terence K.M.} and Ho, {P. L.} and Woo, {Patrick C.Y.} and Yuen, {K. Y.} and Chau, {P. Y.}",

year = "2002",

doi = "10.1128/AAC.46.4.1132-1135.2002",

language = "English",

volume = "46",

pages = "1132--1135",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "4",

}

TY - JOUR

T1 - Cloning and expression of class A β-lactamase gene blaABPS in Burkholderia pseudomallei

AU - Cheung, Terence K.M.

AU - Ho, P. L.

AU - Woo, Patrick C.Y.

AU - Yuen, K. Y.

AU - Chau, P. Y.

PY - 2002

Y1 - 2002

N2 - The β-lactamase gene blaABPS in Burkholderia pseudomallei was cloned and expressed in Escherichia coli. BPS-1 is a cephalosporinase with an isoelectric point of 7.7. Sequence analysis of BPS-1 revealed conserved motifs typical of class A β-lactamases and a relationship to the PenA (in B. cepacia) and BlaI (in Yersinia enterocolitica) lineages.

AB - The β-lactamase gene blaABPS in Burkholderia pseudomallei was cloned and expressed in Escherichia coli. BPS-1 is a cephalosporinase with an isoelectric point of 7.7. Sequence analysis of BPS-1 revealed conserved motifs typical of class A β-lactamases and a relationship to the PenA (in B. cepacia) and BlaI (in Yersinia enterocolitica) lineages.

UR - http://www.scopus.com/inward/record.url?scp=0036210598&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036210598&partnerID=8YFLogxK

U2 - 10.1128/AAC.46.4.1132-1135.2002

DO - 10.1128/AAC.46.4.1132-1135.2002

M3 - Article

C2 - 11897607

AN - SCOPUS:0036210598

SN - 0066-4804

VL - 46

SP - 1132

EP - 1135

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 4