Characterization of a Tsukamurella pseudo-outbreak by phenotypic tests, 16S rRNA sequencing, pulsed-field gel electrophoresis, and metabolic footprinting

Kelvin K.W. To; Ami M.Y. Fung; Jade L.L. Teng; Shirly O.T. Curreem; Kim Chung Lee; Kwok Yung Yuen; Ching Wan Lam; Susanna K.P. Lau; Patrick C.Y. Woo

doi:10.1128/JCM.02845-12

Characterization of a Tsukamurella pseudo-outbreak by phenotypic tests, 16S rRNA sequencing, pulsed-field gel electrophoresis, and metabolic footprinting

Kelvin K.W. To, Ami M.Y. Fung, Jade L.L. Teng, Shirly O.T. Curreem, Kim Chung Lee, Kwok Yung Yuen, Ching Wan Lam, Susanna K.P. Lau, Patrick C.Y. Woo

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

We report a pseudo-outbreak of Tsukamurella due to improperly wrapped scissors used for processing of tissue specimens. A polyphasic approach, involving biochemical, genetic, and metabolomic techniques, was used in the laboratory investigation. This report highlights that early recognition of pseudo-outbreaks is important in preventing unnecessary and incorrect treatment of patients.

Original language	English
Pages (from-to)	334-338
Number of pages	5
Journal	Journal of Clinical Microbiology
Volume	51
Issue number	1
DOIs	https://doi.org/10.1128/JCM.02845-12
Publication status	Published - Jan 2013
Externally published	Yes

ASJC Scopus Subject Areas

Microbiology (medical)

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To, K. K. W., Fung, A. M. Y., Teng, J. L. L., Curreem, S. O. T., Lee, K. C., Yuen, K. Y., Lam, C. W., Lau, S. K. P., & Woo, P. C. Y. (2013). Characterization of a Tsukamurella pseudo-outbreak by phenotypic tests, 16S rRNA sequencing, pulsed-field gel electrophoresis, and metabolic footprinting. Journal of Clinical Microbiology, 51(1), 334-338. https://doi.org/10.1128/JCM.02845-12

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AU - To, Kelvin K.W.

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AU - Teng, Jade L.L.

AU - Curreem, Shirly O.T.

AU - Lee, Kim Chung

AU - Yuen, Kwok Yung

AU - Lam, Ching Wan

AU - Lau, Susanna K.P.

AU - Woo, Patrick C.Y.

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Characterization of a Tsukamurella pseudo-outbreak by phenotypic tests, 16S rRNA sequencing, pulsed-field gel electrophoresis, and metabolic footprinting

Abstract

ASJC Scopus Subject Areas

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