Application of a dual target PCR-high resolution melting (HRM) method for rapid nontuberculous mycobacteria identification

Jonathan HK Chen; Vincent CC Cheng; Kevin KK She; Wing Cheong Yam; Kwok Yung Yuen

doi:10.1016/j.mimet.2016.11.004

Application of a dual target PCR-high resolution melting (HRM) method for rapid nontuberculous mycobacteria identification

Jonathan HK Chen, Vincent CC Cheng, Kevin KK She, Wing Cheong Yam, Kwok Yung Yuen

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Species differentiation of nontuberculous mycobacteria (NTM) has long been a difficult task in clinical laboratories. This study demonstrated and evaluated a simple and cost-effective method using the real-time PCR with high-resolution melting (PCR-HRM) analysis technique, which could differentiate at least 14 different medically related NTM.

Original language	English
Pages (from-to)	1-3
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	132
DOIs	https://doi.org/10.1016/j.mimet.2016.11.004
Publication status	Published - Jan 1 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2016

ASJC Scopus Subject Areas

Microbiology
Molecular Biology
Microbiology (medical)

Keywords

High-resolution melting
Nontuberculous mycobacteria

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10.1016/j.mimet.2016.11.004

Cite this

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abstract = "Species differentiation of nontuberculous mycobacteria (NTM) has long been a difficult task in clinical laboratories. This study demonstrated and evaluated a simple and cost-effective method using the real-time PCR with high-resolution melting (PCR-HRM) analysis technique, which could differentiate at least 14 different medically related NTM.",

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AU - Cheng, Vincent CC

AU - She, Kevin KK

AU - Yam, Wing Cheong

AU - Yuen, Kwok Yung

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Species differentiation of nontuberculous mycobacteria (NTM) has long been a difficult task in clinical laboratories. This study demonstrated and evaluated a simple and cost-effective method using the real-time PCR with high-resolution melting (PCR-HRM) analysis technique, which could differentiate at least 14 different medically related NTM.

AB - Species differentiation of nontuberculous mycobacteria (NTM) has long been a difficult task in clinical laboratories. This study demonstrated and evaluated a simple and cost-effective method using the real-time PCR with high-resolution melting (PCR-HRM) analysis technique, which could differentiate at least 14 different medically related NTM.

KW - High-resolution melting

KW - Nontuberculous mycobacteria

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ER -

Application of a dual target PCR-high resolution melting (HRM) method for rapid nontuberculous mycobacteria identification

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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Cite this