Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43

Xiao Yan Che; Li Wen Qiu; Zhi Yong Liao; Ya Di Wang; Kun Wen; Yu Xian Pan; Wei Hao; Ya Bo Mei; Vincent C.C. Cheng; Kwok Yung Yuen

doi:10.1086/430355

Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43

Xiao Yan Che, Li Wen Qiu, Zhi Yong Liao, Ya Di Wang, Kun Wen, Yu Xian Pan, Wei Hao, Ya Bo Mei, Vincent C.C. Cheng, Kwok Yung Yuen

Research output: Contribution to journal › Article › peer-review

139 Citations (Scopus)

Abstract

Cross-reactivity between antibodies to different human coronaviruses (HCoVs) has not been systematically studied. By use of Western blot analysis, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA), antigenic cross-reactivity between severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) and 2 HCoVs (229E and OC43) was demonstrated in immunized animals and human serum. In 5 of 11 and 10 of 11 patients with SARS, paired serum samples showed a ≥4-fold increase in antibody titers against HCoV-229E and HCoV-OC43, respectively, by IFA. Overall, serum samples from convalescent patients who had SARS had a 1-way cross-reactivity with the 2 known HCoVs. Antigens of SARS-CoV and HCoV-OC43 were more cross-reactive than were those of SARS-CoV and HCoV-229E.

Original language	English
Pages (from-to)	2033-2037
Number of pages	5
Journal	Journal of Infectious Diseases
Volume	191
Issue number	12
DOIs	https://doi.org/10.1086/430355
Publication status	Published - Jun 15 2005
Externally published	Yes

ASJC Scopus Subject Areas

Immunology and Allergy
Infectious Diseases

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title = "Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43",

abstract = "Cross-reactivity between antibodies to different human coronaviruses (HCoVs) has not been systematically studied. By use of Western blot analysis, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA), antigenic cross-reactivity between severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) and 2 HCoVs (229E and OC43) was demonstrated in immunized animals and human serum. In 5 of 11 and 10 of 11 patients with SARS, paired serum samples showed a ≥4-fold increase in antibody titers against HCoV-229E and HCoV-OC43, respectively, by IFA. Overall, serum samples from convalescent patients who had SARS had a 1-way cross-reactivity with the 2 known HCoVs. Antigens of SARS-CoV and HCoV-OC43 were more cross-reactive than were those of SARS-CoV and HCoV-229E.",

author = "Che, {Xiao Yan} and Qiu, {Li Wen} and Liao, {Zhi Yong} and Wang, {Ya Di} and Kun Wen and Pan, {Yu Xian} and Wei Hao and Mei, {Ya Bo} and Cheng, {Vincent C.C.} and Yuen, {Kwok Yung}",

year = "2005",

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doi = "10.1086/430355",

language = "English",

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AU - Che, Xiao Yan

AU - Qiu, Li Wen

AU - Liao, Zhi Yong

AU - Wang, Ya Di

AU - Wen, Kun

AU - Pan, Yu Xian

AU - Hao, Wei

AU - Mei, Ya Bo

AU - Cheng, Vincent C.C.

AU - Yuen, Kwok Yung

PY - 2005/6/15

Y1 - 2005/6/15

N2 - Cross-reactivity between antibodies to different human coronaviruses (HCoVs) has not been systematically studied. By use of Western blot analysis, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA), antigenic cross-reactivity between severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) and 2 HCoVs (229E and OC43) was demonstrated in immunized animals and human serum. In 5 of 11 and 10 of 11 patients with SARS, paired serum samples showed a ≥4-fold increase in antibody titers against HCoV-229E and HCoV-OC43, respectively, by IFA. Overall, serum samples from convalescent patients who had SARS had a 1-way cross-reactivity with the 2 known HCoVs. Antigens of SARS-CoV and HCoV-OC43 were more cross-reactive than were those of SARS-CoV and HCoV-229E.

AB - Cross-reactivity between antibodies to different human coronaviruses (HCoVs) has not been systematically studied. By use of Western blot analysis, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA), antigenic cross-reactivity between severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) and 2 HCoVs (229E and OC43) was demonstrated in immunized animals and human serum. In 5 of 11 and 10 of 11 patients with SARS, paired serum samples showed a ≥4-fold increase in antibody titers against HCoV-229E and HCoV-OC43, respectively, by IFA. Overall, serum samples from convalescent patients who had SARS had a 1-way cross-reactivity with the 2 known HCoVs. Antigens of SARS-CoV and HCoV-OC43 were more cross-reactive than were those of SARS-CoV and HCoV-229E.

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Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43

Abstract

ASJC Scopus Subject Areas

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