Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1)

K. H. Chan; S. T. Lai; L. L.M. Poon; Y. Guan; K. Y. Yuen; J. S.M. Peiris

doi:10.1016/j.jcv.2009.05.034

Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1)

K. H. Chan, S. T. Lai, L. L.M. Poon, Y. Guan, K. Y. Yuen, J. S.M. Peiris

Research output: Contribution to journal › Article › peer-review

107 Citations (Scopus)

Abstract

Background: A novel swine origin influenza virus (S-OIV) (H1N1) is spreading worldwide and threatens to become pandemic. Objectives: Determine analytical sensitivity of selected commercially available rapid influenza antigen detection tests in detecting S-OIV H1N1. Study design: Serial dilutions of two S-OIV isolates, one seasonal influenza A (H1N1) isolate and a nasopharyngeal aspirate from a patient with S-OIV disease were tested in five commercially available influenza antigen detection tests and by virus isolation in cell culture. Viral M gene copy number was determined by quantitative PCR methods. Results: The analytical sensitivity of the five influenza antigen detection tests for S-OIV (tissue culture infectious dose 50 (TCID₅₀) log₁₀ 3.3-4.7 was comparable with that of seasonal influenza (TCID₅₀ log₁₀ 4.0-4.5). Conclusion: The analytical sensitivity of the selected influenza A antigen detection tests for detection of S-IOV was comparable with that of seasonal influenza H1N1.

Original language	English
Pages (from-to)	205-207
Number of pages	3
Journal	Journal of Clinical Virology
Volume	45
Issue number	3
DOIs	https://doi.org/10.1016/j.jcv.2009.05.034
Publication status	Published - Jul 2009
Externally published	Yes

ASJC Scopus Subject Areas

Virology
Infectious Diseases

Keywords

Antigen detection
Diagnostics
H1N1
Influenza
Pandemic
Point-of care

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10.1016/j.jcv.2009.05.034

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title = "Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1)",

abstract = "Background: A novel swine origin influenza virus (S-OIV) (H1N1) is spreading worldwide and threatens to become pandemic. Objectives: Determine analytical sensitivity of selected commercially available rapid influenza antigen detection tests in detecting S-OIV H1N1. Study design: Serial dilutions of two S-OIV isolates, one seasonal influenza A (H1N1) isolate and a nasopharyngeal aspirate from a patient with S-OIV disease were tested in five commercially available influenza antigen detection tests and by virus isolation in cell culture. Viral M gene copy number was determined by quantitative PCR methods. Results: The analytical sensitivity of the five influenza antigen detection tests for S-OIV (tissue culture infectious dose 50 (TCID50) log10 3.3-4.7 was comparable with that of seasonal influenza (TCID50 log10 4.0-4.5). Conclusion: The analytical sensitivity of the selected influenza A antigen detection tests for detection of S-IOV was comparable with that of seasonal influenza H1N1.",

keywords = "Antigen detection, Diagnostics, H1N1, Influenza, Pandemic, Point-of care",

author = "Chan, {K. H.} and Lai, {S. T.} and Poon, {L. L.M.} and Y. Guan and Yuen, {K. Y.} and Peiris, {J. S.M.}",

year = "2009",

month = jul,

doi = "10.1016/j.jcv.2009.05.034",

language = "English",

volume = "45",

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journal = "Journal of Clinical Virology",

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T1 - Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1)

AU - Chan, K. H.

AU - Lai, S. T.

AU - Poon, L. L.M.

AU - Guan, Y.

AU - Yuen, K. Y.

AU - Peiris, J. S.M.

PY - 2009/7

Y1 - 2009/7

N2 - Background: A novel swine origin influenza virus (S-OIV) (H1N1) is spreading worldwide and threatens to become pandemic. Objectives: Determine analytical sensitivity of selected commercially available rapid influenza antigen detection tests in detecting S-OIV H1N1. Study design: Serial dilutions of two S-OIV isolates, one seasonal influenza A (H1N1) isolate and a nasopharyngeal aspirate from a patient with S-OIV disease were tested in five commercially available influenza antigen detection tests and by virus isolation in cell culture. Viral M gene copy number was determined by quantitative PCR methods. Results: The analytical sensitivity of the five influenza antigen detection tests for S-OIV (tissue culture infectious dose 50 (TCID50) log10 3.3-4.7 was comparable with that of seasonal influenza (TCID50 log10 4.0-4.5). Conclusion: The analytical sensitivity of the selected influenza A antigen detection tests for detection of S-IOV was comparable with that of seasonal influenza H1N1.

AB - Background: A novel swine origin influenza virus (S-OIV) (H1N1) is spreading worldwide and threatens to become pandemic. Objectives: Determine analytical sensitivity of selected commercially available rapid influenza antigen detection tests in detecting S-OIV H1N1. Study design: Serial dilutions of two S-OIV isolates, one seasonal influenza A (H1N1) isolate and a nasopharyngeal aspirate from a patient with S-OIV disease were tested in five commercially available influenza antigen detection tests and by virus isolation in cell culture. Viral M gene copy number was determined by quantitative PCR methods. Results: The analytical sensitivity of the five influenza antigen detection tests for S-OIV (tissue culture infectious dose 50 (TCID50) log10 3.3-4.7 was comparable with that of seasonal influenza (TCID50 log10 4.0-4.5). Conclusion: The analytical sensitivity of the selected influenza A antigen detection tests for detection of S-IOV was comparable with that of seasonal influenza H1N1.

KW - Antigen detection

KW - Diagnostics

KW - H1N1

KW - Influenza

KW - Pandemic

KW - Point-of care

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Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1)

Abstract

ASJC Scopus Subject Areas

Keywords

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