A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors

Leo L.M. Poon; Bonnie W.Y. Wong; Kwok Hung Chan; Cynthia S.W. Leung; Kwok Yung Yuen; Yi Guan; Joseph S.M. Peiris

doi:10.1016/j.jcv.2003.12.007

A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors

Leo L.M. Poon, Bonnie W.Y. Wong, Kwok Hung Chan, Cynthia S.W. Leung, Kwok Yung Yuen, Yi Guan, Joseph S.M. Peiris

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to PCR inhibitors or faulty RNA extraction, we have further modified this one step RT-PCR assay for simultaneous detection of severe acute respiratory syndrome (SARS) coronavirus (CoV) and 18S ribosomal RNA (rRNA) as an internal positive control.

Original language	English
Pages (from-to)	214-217
Number of pages	4
Journal	Journal of Clinical Virology
Volume	30
Issue number	3
DOIs	https://doi.org/10.1016/j.jcv.2003.12.007
Publication status	Published - Jul 2004
Externally published	Yes

ASJC Scopus Subject Areas

Virology
Infectious Diseases

Keywords

Early diagnosis
Quantitative RT-PCR
SARS coronavirus

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10.1016/j.jcv.2003.12.007

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abstract = "In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to PCR inhibitors or faulty RNA extraction, we have further modified this one step RT-PCR assay for simultaneous detection of severe acute respiratory syndrome (SARS) coronavirus (CoV) and 18S ribosomal RNA (rRNA) as an internal positive control.",

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AU - Poon, Leo L.M.

AU - Wong, Bonnie W.Y.

AU - Chan, Kwok Hung

AU - Leung, Cynthia S.W.

AU - Yuen, Kwok Yung

AU - Guan, Yi

AU - Peiris, Joseph S.M.

PY - 2004/7

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AB - In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to PCR inhibitors or faulty RNA extraction, we have further modified this one step RT-PCR assay for simultaneous detection of severe acute respiratory syndrome (SARS) coronavirus (CoV) and 18S ribosomal RNA (rRNA) as an internal positive control.

KW - Early diagnosis

KW - Quantitative RT-PCR

KW - SARS coronavirus

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A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors

Abstract

ASJC Scopus Subject Areas

Keywords

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