TY - JOUR
T1 - A novel peptide with potent and broad-spectrum antiviral activities against multiple respiratory viruses
AU - Zhao, Hanjun
AU - Zhou, Jie
AU - Zhang, Ke
AU - Chu, Hin
AU - Liu, Dabin
AU - Poon, Vincent Kwok Man
AU - Chan, Chris Chung Sing
AU - Leung, Ho Chuen
AU - Fai, Ng
AU - Lin, Yong Ping
AU - Zhang, Anna Jin Xia
AU - Jin, Dong Yan
AU - Yuen, Kwok Yung
AU - Zheng, Bo Jian
PY - 2016/2/25
Y1 - 2016/2/25
N2 - A safe, potent and broad-spectrum antiviral is urgently needed to combat emerging respiratory viruses. In light of the broad antiviral activity of β-defensins, we tested the antiviral activity of 11 peptides derived from mouse β-defensin-4 and found that a short peptide, P9, exhibited potent and broad-spectrum antiviral effects against multiple respiratory viruses in vitro and in vivo, including influenza A virus H1N1, H3N2, H5N1, H7N7, H7N9, SARS-CoV and MERS-CoV. The antiviral activity of P9 was attributed to its high-affinity binding to viral glycoproteins, as well as the abundance of basic amino acids in its composition. After binding viral particles through viral surface glycoproteins, P9 entered into cells together with the viruses via endocytosis and prevented endosomal acidification, which blocked membrane fusion and subsequent viral RNA release. This study has paved the avenue for developing new prophylactic and therapeutic agents with broad-spectrum antiviral activities.
AB - A safe, potent and broad-spectrum antiviral is urgently needed to combat emerging respiratory viruses. In light of the broad antiviral activity of β-defensins, we tested the antiviral activity of 11 peptides derived from mouse β-defensin-4 and found that a short peptide, P9, exhibited potent and broad-spectrum antiviral effects against multiple respiratory viruses in vitro and in vivo, including influenza A virus H1N1, H3N2, H5N1, H7N7, H7N9, SARS-CoV and MERS-CoV. The antiviral activity of P9 was attributed to its high-affinity binding to viral glycoproteins, as well as the abundance of basic amino acids in its composition. After binding viral particles through viral surface glycoproteins, P9 entered into cells together with the viruses via endocytosis and prevented endosomal acidification, which blocked membrane fusion and subsequent viral RNA release. This study has paved the avenue for developing new prophylactic and therapeutic agents with broad-spectrum antiviral activities.
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U2 - 10.1038/srep22008
DO - 10.1038/srep22008
M3 - Article
C2 - 26911565
AN - SCOPUS:84959422192
SN - 2045-2322
VL - 6
JO - Scientific Reports
JF - Scientific Reports
M1 - 22008
ER -